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. 2020 Jul 16;11(7):538. doi: 10.1038/s41419-020-2727-2

Fig. 5. Ongoing transcription is required for compromising DNA replication by the ISR.

Fig. 5

a Cells were pre-treated with CDK9 inhibitors (25 μM DRB, 10 μM LDC067) for 1 h prior to labeling with CldU (30 min) and IdU (60 min) in the presence of CDK9i and Thap (4 μM). Cells were then harvested for DNA fiber analysis. b U2OS cells were treated with CDK9i (25 μM DRB, 10 μM LDC067) and BEPP (10 μM) for 1 h and then labeled with CldU (30 min) and IdU (60 min) with both CDK9i and BEPP prior to analysis. c, d Representative DNA fiber tracks of cells treated with CDK9i/Thap (c) or CDK9i/BEPP (d) visualized via immunostaining of CldU (red) and IdU (green). e, f IdU tracks of cells treated with CDK9i and Thap (e) or CDK9i and BEPP (f) were used to measure fork progression and presented as box plots (5–95 percentile whiskers). One representative plot from three independent experiments shown. See Supplementary Fig. S5A–D.