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. 2020 Jul 16;10:11754. doi: 10.1038/s41598-020-68254-1

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ex vivo porcine corneal permeability to fluorescein (A i), rhodamine B (A ii) and dextran blue (A iii). Results are displayed as bar charts with mean and standard error (error bars). (B i–iv) fluorescent microscopy images of anterior stroma and epithelium after fluorescein (B i, ii) and rhodamine B (B iii, iv) application, without (B i, iii) and with (B ii, iv) DAPI staining of cell nuclei. Scale bar 100 µm. Graphs represents data from 3 independent experiments for rhodamine, 4 repeats for fluorescein and 5 repeats for dextran blue.