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. 2020 Jul 16;10:11754. doi: 10.1038/s41598-020-68254-1

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ZO-1 staining for epithelial tight junctions in corneal sections at baseline (A–C) and after 60 min (D–F) in the model with HBSS appled. (A, D) combined images; (B, E) ZO-1 staining in green; (C, F) DAPI-stained nuclei. “e” marks corneal epithelium; “s” marks corneal stroma; scale bar 50 µm.