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. 2020 Jul 16;10:11754. doi: 10.1038/s41598-020-68254-1

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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siCASP2 adsorption to the corneal epithelium demonstrated by FISH staining. 10 µg siCASP2 was applied to the corneal surface and left on for the duration of the experiment (A) or washed off after 2 min (B) to simulate lacrimal drainage. Green staining represents siCASP2 and blue represents DAPI (nucleic acid) staining. Background fluorescence is seen in (A i) and (B i) images of cornea 60 and 240 min after HBSS application. “e” marks corneal epithelium; “s” marks corneal stroma; scale bar 50 µm.