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. 2020 Jul 16;10:11754. doi: 10.1038/s41598-020-68254-1

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) representative H&E-stained human corneal tissue from the assays in (B–D): (i), human peripheral cornea; (ii), human sclera; (iii), human central cornea after posterior lamellae removal. (B–D) Penetration of siCASP2 through and adsorption to human cornea and sclera over 60 min after siCASP2 was applied to the epithelial surface and washed off after 2 min. Results are displayed as bar charts with mean and standard error (error bars). (D) is displayed with a split y axis. “ep” corneal epithelium; “s” corneal stroma; “en” corneal endothelium; “sc” sclera; “co” conjunctiva; scale bar 100 µm. (B, C) represent data from n = 2 independent experiments; (D) represents data from n = 3 independent experiments.