Figure 4.

The physically-based CAP treatment effectively inhibited the growth of glioblastoma cells. The CAP treatment was performed for 1 min (a), 2 min (b), 4 min (c), and 8 min (d). The treatment was performed on the bottom of the 96-well plate, targeting the well 6D. For each case, 100 μL/well of U87MG (12 × 10⁴ cell/mL) were seeded in 96-well plate and cultured for 7 h before the treatment. The cells were cultured for 3 days before the final MTT assay. The normalized cell viability was obtained by using the protocols shown in Fig. 2. In each case, the mean value and the s.d. of the normalized cell viability were presented in the left panel and the right panel, respectively. Results were presented as the mean ± s.d. of the experiments repeated for 4 times. The original data were shown in Supplementary Fig. S2.