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. 2020 Jul 16;10:11788. doi: 10.1038/s41598-020-68585-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The growth of bubbles on the CAP-treated U87MG cells. Two cases were shown here. The photos were taken after 4 min of CAP treatment. The arrow with a specific color marked a specific bubble on the cytoplasm membrane. 1.5 mL of U87MG cells (7.5 × 10⁴ cell/mL) were seeded in 35 mm dishes and cultured for 24 h in the incubator. ‘ + x min’ meant the photo was taken at x min after the CAP treatment. The scale bar was 50 μm (black). In each row, the photos were taken in situ. All photos were taken by using a Nikon TS100 inverted phase-contrast microscope.