Fig. 1.

Early surge in phagocytic cells in the grafts of granulocyte colony‐stimulating factor (G‐CSF)/rapamycin‐treated and transplanted mice. Diabetic C57BL/6 mice were transplanted with Balb/c‐derived pancreatic islets and treated for 30 days with G‐CSF/rapamycin. Grafts and spleens were harvested and cells were collected and detected by flow cytometry. One representative dot‐plot (out of four per group) of flow cytometry staining of graft‐infiltrating F4/80⁺CD11b⁺ macrophages gated on CD11c⁻ cells [untreated mice: upper left panel (a), treated mice: upper right panel (a)] and dendritic cells (DC) gated on live cells [untreated mice: lower left panel (a), treated mice: lower right panel (a)] infiltrating the spleens (b) and the grafts (c) of G‐CSF/rapamycin‐treated (closed circles) and untreated (open circles) diabetic C57BL/6 mice over time. Data are presented as mean ± standard error of the mean (s.e.m.), n = 4–6/group, **P < 0·01, n.s. = non‐significant, Mann–Whitney test.