Fig. 8.
Different methods for regulatory T cell (Treg) differentiation in the co‐culture of naive T and nTreg cells. (a) In the co‐culture groups, CD25+forkhead box protein 3 (FoxP3+) cells were compared in two differentiation groups, soluble transforming growth factor‐β (S‐TGF) group and the N‐acetyl puromycin and SR1555 (N‐Ac/SR) group, under inflammatory and non‐inflammatory conditions [one‐way analysis of variance (anova)]. (b) In the co‐culture groups, the levels of interleukin (IL)‐17A, IL‐10 and interferon (IFN)‐γ were measured in the two groups after 10 days in the non‐inflammatory condition (one‐way anova). (c) Suppressive activity was analyzed by carboxyfluorescein succinimidyl ester (CFSE) after 10 days. A comparison was made between the N‐Ac/SR and S‐TGF protocols (one‐way anova). The results are presented as mean ± standard deviation. *P < 0·05; **P < 0·001; ***P = 0·0002; ****P < 0·0001; three to four donors in the independent experiments.