Table 1. mRNA levels of genes associated with the pan JAK-STAT pathway in primary human basal epithelial cells.
scRNA-seq data were extracted from the study by Ziegler and colleagues(Ziegler, 2020). The human bronchial cell line (BEAS-2B) and airway basal cells from human donors had been exposed to interferons (IFNα2 and IFNγ) and cytokines (IL4 and IL17A). scRNA-seq libraries were generated with 15,000 cells. mRNA levels for genes in JAK/STAT signaling pathway were collected from the data and averages of independent biological replicates were normalized to the value of untreated group. Genes that were regulated more than 3-fold by interferons and cytokines are marked in red and highlighted colors.
| Cells | BEAS cell | Human1334 | Human2344 | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| N | 12 | 3 | 3 | 3 | 3 | 9 | 3 | 3 | 3 | 3 | 12 | 3 | 3 | 3 | 3 |
| drug (10ng/mL) | Untreated | IFNa2 | IFNg | IL4 | IL17A | Untreated | IFNa2 | IFNg | IL4 | IL17A | Untreated | IFNa2 | IFNg | IL4 | IL17A |
| ACE2 | 1.00 | 2.18 | 6.59 | 0.00 | 0.00 | 1.00 | 17.53 | 8.40 | 0.96 | 1.04 | 1.00 | 5.69 | 3.42 | 2.30 | 0.58 |
| TMPRSS2 | 1.00 | 0.19 | 0.59 | 0.30 | 0.51 | 1.00 | 0.52 | 0.53 | 2.42 | 1.03 | 1.00 | 0.74 | 0.71 | 1.38 | 1.49 |
| IFNAR1 | 1.00 | 0.78 | 1.01 | 0.87 | 1.11 | 1.00 | 0.76 | 0.61 | 0.86 | 0.97 | 1.00 | 0.79 | 0.56 | 0.76 | 0.66 |
| IFNAR2 | 1.00 | 1.26 | 1.72 | 0.90 | 1.20 | 1.00 | 1.05 | 1.33 | 0.90 | 0.75 | 1.00 | 0.80 | 1.06 | 0.70 | 0.94 |
| IFNGR1 | 1.00 | 1.08 | 0.74 | 0.52 | 1.32 | 1.00 | 1.01 | 0.74 | 0.74 | 1.70 | 1.00 | 0.97 | 0.64 | 0.94 | 1.20 |
| IL4R | 1.00 | 1.36 | 1.41 | 1.23 | 1.22 | 1.00 | 0.86 | 0.87 | 0.78 | 1.19 | 1.00 | 0.86 | 1.31 | 1.29 | 1.07 |
| IL12RB1 | 1.00 | 0.69 | 0.54 | 0.26 | 0.47 | 1.00 | 2.30 | 2.74 | 2.05 | 3.01 | 1.00 | 1.56 | 2.55 | 3.81 | 2.11 |
| IL12RB2 | 1.00 | 0.00 | 1.47 | 1.36 | 0.62 | 1.00 | 0.37 | 1.99 | 1.10 | 1.11 | 1.00 | 3.30 | 4.49 | 5.57 | 0.00 |
| IL13RA1 | 1.00 | 1.06 | 1.04 | 0.50 | 1.05 | 1.00 | 1.59 | 1.32 | 1.30 | 2.39 | 1.00 | 1.95 | 1.87 | 1.11 | 2.10 |
| IL13RA2 | 1.00 | 0.00 | 0.00 | 33.6 | 14.14 | 1.00 | 1.73 | 1.78 | 5.79 | 1.69 | 1.00 | 1.02 | 1.14 | 3.04 | 1.95 |
| IL15RA | 1.00 | 1.23 | 3.00 | 1.02 | 1.03 | 1.00 | 3.37 | 7.94 | 0.79 | 0.88 | 1.00 | 3.21 | 8.89 | 1.12 | 0.87 |
| IL17RA | 1.00 | 1.56 | 0.90 | 2.73 | 1.60 | 1.00 | 0.40 | 2.01 | 2.17 | 1.27 | 1.00 | 0.78 | 0.80 | 0.46 | 0.29 |
| IL17RC | 1.00 | 1.31 | 1.61 | 0.71 | 1.35 | 1.00 | 1.44 | 1.66 | 1.13 | 1.25 | 1.00 | 1.60 | 1.15 | 1.39 | 1.44 |
| STAT1 | 1.00 | 3.28 | 4.77 | 0.52 | 0.78 | 1.00 | 19.48 | 10.73 | 1.05 | 1.00 | 1.00 | 30.08 | 17.39 | 1.22 | 1.41 |
| STAT2 | 1.00 | 1.89 | 1.94 | 0.53 | 0.68 | 1.00 | 5.78 | 3.25 | 0.92 | 1.06 | 1.00 | 8.96 | 4.96 | 1.15 | 1.10 |
| STAT3 | 1.00 | 1.11 | 1.17 | 0.50 | 0.78 | 1.00 | 1.11 | 1.14 | 0.71 | 1.02 | 1.00 | 1.30 | 1.65 | 1.32 | 1.35 |
| STAT4 | 1.00 | 1.13 | 0.53 | 0.07 | 1.12 | 1.00 | 1.20 | 1.03 | 0.76 | 1.00 | 1.00 | 0.75 | 0.52 | 1.26 | 2.61 |
| STAT5A | 1.00 | 1.17 | 0.86 | 0.22 | 0.54 | 1.00 | 1.65 | 4.90 | 0.70 | 1.88 | 1.00 | 3.97 | 2.91 | 0.89 | 1.23 |
| STAT5B | 1.00 | 0.79 | 0.69 | 0.38 | 0.63 | 1.00 | 2.61 | 2.26 | 1.52 | 2.08 | 1.00 | 1.37 | 2.51 | 2.59 | 1.48 |
| STAT6 | 1.00 | 0.97 | 0.88 | 0.39 | 0.53 | 1.00 | 1.18 | 0.67 | 0.83 | 1.04 | 1.00 | 1.13 | 0.93 | 1.27 | 1.26 |
| JAK1 | 1.00 | 1.06 | 1.07 | 1.55 | 1.40 | 1.00 | 1.22 | 1.54 | 1.13 | 1.21 | 1.00 | 1.22 | 0.94 | 0.85 | 1.02 |
| JAK2 | 1.00 | 1.73 | 3.62 | 0.68 | 1.28 | 1.00 | 2.11 | 3.16 | 1.05 | 1.24 | 1.00 | 4.11 | 2.86 | 1.28 | 0.59 |
| JAK3 | 1.00 | 1.00 | 0.74 | 0.53 | 0.68 | 1.00 | 2.01 | 2.31 | 0.83 | 1.63 | 1.00 | 1.76 | 2.72 | 2.09 | 1.36 |
| TYK2 | 1.00 | 0.88 | 0.94 | 0.64 | 1.06 | 1.00 | 1.00 | 0.79 | 1.09 | 1.27 | 1.00 | 1.01 | 1.12 | 1.37 | 1.32 |
| LCN2 | 1.00 | 1.13 | 0.96 | 0.93 | 2.73 | 1.00 | 0.76 | 0.61 | 0.58 | 1.72 | 1.00 | 1.39 | 1.09 | 1.02 | 2.40 |
| FABP5 | 1.00 | 1.12 | 1.10 | 1.65 | 1.16 | 1.00 | 1.30 | 1.17 | 0.96 | 0.96 | 1.00 | 1.55 | 1.00 | 0.47 | 0.57 |
| AQP3 | 1.00 | 0.89 | 0.86 | 0.47 | 0.70 | 1.00 | 0.85 | 0.85 | 0.72 | 0.85 | 1.00 | 1.01 | 0.64 | 1.26 | 0.97 |
| SLC25A1 | 1.00 | 1.76 | 2.87 | 2.05 | 3.07 | 1.00 | 1.12 | 1.72 | 1.93 | 2.06 | 1.00 | 1.76 | 1.81 | 3.02 | 2.62 |
| SOCS2 | 1.00 | 1.43 | 1.13 | 0.65 | 1.47 | 1.00 | 1.21 | 0.45 | 0.96 | 0.90 | 1.00 | 0.64 | 0.69 | 2.64 | 2.18 |
| SOCS3 | 1.00 | 1.86 | 2.78 | 0.63 | 1.90 | 1.00 | 0.86 | 2.94 | 1.18 | 2.32 | 1.00 | 0.95 | 8.43 | 3.65 | 1.94 |
| BCL6 | 1.00 | 0.77 | 0.88 | 0.80 | 1.09 | 1.00 | 0.76 | 1.68 | 0.84 | 0.80 | 1.00 | 0.99 | 0.88 | 1.02 | 0.66 |
| CISH | 1.00 | 1.34 | 1.23 | 9.76 | 1.34 | 1.00 | 0.39 | 0.81 | 4.72 | 0.94 | 1.00 | 0.81 | 1.23 | 8.07 | 1.05 |