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Visualization of the neuronal cytoplasm and plasma membrane. (A,B) Scheme (A) and confocal pictures (B, maximal projections) of an immature neuron (DIV5) expressing pHluoTM (green) and mCherry (red). PHluoTM – a 39 KDa type I transmembrane protein derived from superecliptic (pH-sensitive) GFP – selectively fluoresces and marks the plasma membrane (arrows). mCherry, a 27KDa soluble protein, distributes throughout the cytoplasm (arrowheads). Scale bar 50 or 10 μm.
