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. 2020 Jul 17;9:111. doi: 10.1186/s13756-020-00774-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Sensitivity analysis of m-LAMP detection with serial dilutions of genomic DNA extracted from the MRSA strain. Two sets of primers targeting the femA and mecA genes were simultaneously added to a reaction vessel, and the LoD of m-LAMP for detecting S. aureus and identifying MRSA was analyzed with a lateral flow biosensor. Biosensors 1–8 represent the genomic DNA (methicillin-resistant S. aureus, ATCC43300) amounts of 10 ng, 10 pg, 1 pg, 100 fg, 10 fg, 1 fg and 100 ag per reaction and blank control (DW), respectively. The LoD of the m-LAMP assay for femA and mecA detection was 100 fg of genomic template per reaction