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. 2020 Jul 17;9:111. doi: 10.1186/s13756-020-00774-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Optimization of the amplification time for m-LAMP detection. Different amplification times (a, 20 min; b, 30 min; c, 40 min; d, 50 min) were tested at 63 °C. Biosensors 1, 2, 3, 4, 5, 6, 7, and 8 represent genomic DNA (methicillin-resistant S. aureus, ATCC43300) levels of 10 ng, 10 pg, 1 pg, 100 fg, 10 fg, 1 fg and 100 ag target template per reaction and negative control (DW), respectively. The best sensitivity was observed when the amplification lasted for 40 min (c)