Figure 1.

Long non-coding RNA MNX-AS1 is upregulated in triple negative breast cancer (TNBC) and indicates poor survival outcome in breast cancer patients. (A) The heatmaps of lncRNAs that expressed 5-fold differentially between in triple negative breast cancer and non-triple negative breast cancer. The data were extracted from TCGA datasets Expression levels shown as log₂ transformed intensity relative to the mean value of all samples. (B) The heatmaps of lncRNAs that expressed 5-fold differentially between normal breast tissue and breast cancer tissues. The data were extracted from TCGA datasets. Expression levels shown as log₂ transformed intensity relative to the mean value of all samples. (C) The venn diagram showed the number of overlapping lncRNAs that were upregulated at least 5-folds in triple negative breast cancer and breast cancer samples. (D) LncRNA MNX1-AS1 is significantly upregulated in breast cancer cell lines comparing with normal breast epithelial cell line MCF10A and MNX1-AS1 was upregulated to greatest extend in TNBC cell lines MDA-MB-231 and MDA-MB-468. MNX1-AS1 expression was determined using qRT-PCR and normalized to MCF-10A expression. (E,F) Upregulated levels of MNX1-AS1 in triple negative breast tumors were detected by in situ hybridization (ISH) and associated with significantly poor overall survival (OS) and disease-free survival (DFS) in TNBC patients (n = 95). *P < 0.05, **P < 0.01 and ***P < 0.001.