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. 2020 Jul 10;10:1108. doi: 10.3389/fonc.2020.01108

Figure 4.

Figure 4

MNX1-AS1 promotes progression of TNBC in vivo. (A–C) Tumor image, growth curve and weight of MDA-MB-231 xenografts in nude mice, MDA-MB-231 transfected with control Plko-tet-on-shRNA (as Vector) or Plko-tet-on-shMNX1-AS1 (as shMNX1-AS1) were injected subcutaneously into mammary fat pad of nude mice. Nude mice were fed with doxycycline (doxy) (2 mg/ml) to induce MNX1-AS1 knockdown Xenografts were harvested 30 days after tumor size reach about 200 mm3. Error bars show ± SD (n = 5 per group). (D) Representative image of MNX1-AS1 and p-stat3 staining in paraffin-embedded xenograft sections. (E) Statistical diagram of p-stat3 staining in (D), Bar graphs represent the mean ± SD of three randomly chosen fields. (F,G) Representative image of lung metastasis in different treatments. Bar graphs represent the mean ± SD of metastasis in five lungs. (H) The Schematic representation of the study showed that lncRNA MNX1-AS1 could induce phosphorylation of Stat3 by enhance the interaction between p-JAK and Stat3. Then the phosphorylated Stat3 activated the progression of breast cancer. **P < 0.01 and ***P < 0.001.