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. 2020 Jul 10;11:958. doi: 10.3389/fphar.2020.00958

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Copyright © 2020 Park, Kim, Kim, Yang, Jung, Jang, Seo, Choi and Lee

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of BTS on BDNF and p-CREB/CREB expression and histology in the hippocampus of reserpine-induced depressed mice. Isolated hippocampus lysates were analyzed by western blotting using (A) BDNF and (B) p-CREB/CREB antibodies. β-actin was used as the loading control. Frozen hippocampus sections were analyzed by immunofluorescence using (C) BDNF and (D) p-CREB antibodies. DAPI was used as the loading control. The presented data are representative of three independent experiments. The data represent the mean ± SD (one-way ANOVA: ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. control; *p < 0.05, **p < 0.01, ***p < 0.001 vs. reserpine-only).