Figure 7.

Effect of BTS on the phosphorylation of MAPKs and Akt and NF-κB activity in BV2 cells. (A) BV2 cells were pretreated with BTS for 1 h and then stimulated with LPS (100 ng/ml) for 15 min. Levels of p-JNK, JNK, p-Erk, Erk, p-p38, p38, p-Akt, and Akt were determined by western blotting. β-actin was used as a loading control. (B) BV2 cells were pretreated with BTS for 1 h and then stimulated with LPS (100 ng/ml) for 1 h. Nuclear extracts were analyzed by western blotting using NF-κB p65 antibody. PCNA was used as a loading control. The data represent three independent experiments. The data are expressed as the mean ± SD (one-way ANOVA: ^###p < 0.001 vs. untreated control; *p < 0.05, **p < 0.01, ***p < 0.001 vs. LPS-treated control).