Figure 2.

BAFF attenuated MMP collapse by the incubation with 1% FBS. (A, B) WiL2-NS cells were incubated in the RPMI 1640 medium with 10% or 1% FBS in the presence or absence of 20 ng/ml BAFF. Then, cells were stained with MitoProbe™ JC-1 reagent (A) or DiOC₆ (B) for the detection of MMP. Cells were observed and photographed with 400 × magnification under fluorescence microscope (A, left). Number of high-red fluorescent cells was counted and represented as bar graph with mean ± SD (A, right). Percentage of cells with low fluorescence of DiOC₆ was analyzed by flow cytometry. Mean fluorescence intensity (MFI) in each histogram was analyzed with software FlowJo V10 (B, left). MFI was represented as bar graph with the means ± SD (B, right). All experiments were performed four times. **p < 0.01; significant difference as compared to BAFF-untreated control with 10% FBS. ^#p < 0.05; significant difference as compared to BAFF-untreated control with 1% FBS.