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. 2020 Jul 16;10:11784. doi: 10.1038/s41598-020-68628-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Spleen tyrosine kinase (Syk) is activated by the incubation with 1% FBS or BAFF. (A) WiL2-NS cells were incubated with 20 ng/ml BAFF for 0.5, 1 and 2 h. (B–D) WiL2-NS cells were incubated in the RPMI 1,640 medium with 10% or 1% FBS in the presence or absence of BAY61-3606, Syk inhibitor (C) or BAFF (D). Cell lysates were prepared and western blotting was used to detect each phosphorylated Syk at tyrosine (Y)323, Y352 and Y525/526. All experiments were performed four times. Processing (such as changing brightness and contrast) is applied equally to controls across the entire image. Each band for Syk phosphorylation at Y525/526 was quantified by using ImageJ 1.34. Data in the bar graph represent the means ± SD. *p < 0.05; **p < 0.01; significant difference as compared to BAFF-untreated control with 10% FBS. ^&p < 0.05; significant difference as compared to BAY61-3606-untreated control with 1% FBS (C). ^#p < 0.05; significant difference as compared to BAFF-untreated control with 1% FBS (D).