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. 2020 Jul 16;10:11784. doi: 10.1038/s41598-020-68628-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Syk inhibitor enhanced MMP collapse by the incubation with 1% FBS. (A, B) WiL2-NS cells were incubated in the RPMI 1640 medium with 10% or 1% FBS in the presence or absence of BAY61-3606, Syk inhibitor and/or 20 ng/ml BAFF. Then, cells were stained with MitoProbe™ JC-1 reagent (A) or DiOC₆ (B) for the detection of MMP. Cells were observed and photographed with 400 × magnification under fluorescence microscope (A, top). Number of high-red fluorescent cells was counted and represented as bar graph with mean ± SD (A, bottom). Percentage of cells with low fluorescence of DiOC₆ was analyzed by flow cytometry (B, top). Mean fluorescence intensity (MFI) in each histogram was analyzed with software FlowJo V10. MFI was represented as bar graph with the means ± SD (B, bottom). All experiments were performed four times. **p < 0.01; significant difference as compared to BAFF-untreated and BAY61-3,606-untreated control with 10% FBS. ^#p < 0.05; significant difference as compared to BAFF-untreated and BAY61-3,606-untreated control with 1% FBS. ^&p < 0.05; significant difference as compared to BAFF-treated and BAY61-3,606-untreated control with 1% FBS.