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. 2020 Jul 16;11:3569. doi: 10.1038/s41467-020-17060-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Flow cytometry analysis showing establishment of MAM expression in Daudi cells. The representative histogram shows IgG (negative control) and MAM reactivity following transfection of Daudi cells with either pEF1α-IRES-ZsGreen1 empty vector (mock), EMP3 wild-type (WT) or EMP3 mutant containing a premature stop codon (c.123 C > G, p.Tyr41Ter). b Overexpression of EMP3 in Daudi cells shown by mean fluorescent intensity from three independent experiments; MAM expression is shown as a ratio between MAM reactivity and isotype control (IgG) reactivity in Daudi cells transfected by either mock, EMP3 WT or EMP3 mutant vectors. **P = 0.0011, ***P = 0.001 (two-tailed unpaired student t test). Error bars represent standard error of the mean (SEM). Source data are provided as a Source Data file. c The histogram shows MAM reactivity following transfection of Daudi cells with either mock, wild-type (WT) or EMP3 mutant compared to unstained cells.