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. 2020 Jul 16;10:11824. doi: 10.1038/s41598-020-68796-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Partial least square discriminate analysis (PLS-DA) scores plot of proliferating (Day 0), early differentiating (Day 2) and late differentiating myogenic culture (Day 4). First component versus second component showing the separation of myogenic culture (Day 0) to early (Day 2) and late differentiating myogenic culture (Day 4). Component 1 and 2 explaining about 70% of the data. (B) VIP scores with the corresponding expression heat map. Green and red indicates the low and high metabolites levels respectively.