Table 1.
Global and differential metabolite profiles during myogenic progression of primary human myoblasts.
| S. no | Metabolite name | Mode | Day-0/ versus Day-2 | Day-0/ versus Day-4 | Day-2/ versus Day-4 | |||
|---|---|---|---|---|---|---|---|---|
| Ratio | p value | Ratio | p value | Ratio | P value | |||
| 1 | 3-Methylhistidine | Positive Hilic | 1.957483761 | 0.017058732 | 1.969849069 | 0.01706663 | 1.482804653 | 0.956364752 |
| 2 | 5-Hydroxytryptophan | Positive Hilic | 0.892634278 | 0.501064879 | 0.990409135 | 0.857467453 | 0.539920534 | 0.391557516 |
| 3 | Arginine | Positive Hilic | 2.063405284 | 0.021845753 | 3.019269217 | 0.129708384 | 64.10815532 | 0.510461226 |
| 4 | Carnosine | Positive Hilic | 1.381165782 | 0.231663886 | 0.441840836 | 0.388254784 | 0.925133238 | 0.326132666 |
| 5 | Galactosamine | Positive Hilic | 2.400296514 | 0.011032868 | 3.063427592 | 0.014300249 | 0.063095683 | 0.055447395 |
| 6 | Glutathione (oxidized) | Positive Hilic | 0.155939966 | 0.008695074 | 0.140495964 | 0.040022369 | 18.45594754 | 0.451109586 |
| 7 | Glutathione (reduced) | Positive Hilic | 0.219887217 | 0.478180308 | 0.508236024 | 0.371266079 | 47.07290561 | 0.653463664 |
| 8 | Gly-Val | Positive Hilic | 2.618389502 | 0.265807903 | 2.610573581 | 0.239896174 | 0.06066872 | 0.980335803 |
| 9 | Histidine | Positive Hilic | 0.897460993 | 0.824289642 | 0.68752904 | 0.041119919 | 5.333627958 | 0.590077911 |
| 10 | Kynurenine | Positive Hilic | 1.403423107 | 0.206203297 | 1.877591002 | 0.045644309 | 0.313956092 | 0.206462409 |
| 11 | Lysine | Positive Hilic | 1.07404302 | 0.69878706 | 1.262601926 | 0.439139483 | 41.26193431 | 0.370038949 |
| 12 | N-Acetylhistidine | Positive Hilic | 8.663894138 | 0.010640991 | 12.71642777 | 0.011309978 | 0.043353965 | 0.166758226 |
| 13 | l-Methylhistidine | Positive Hilic | 1.146680443 | 0.006762015 | 0.909198318 | 0.088270644 | 1.191416337 | 0.015088396 |
| 14 | N-Methylisoleucine | Positive Hilic | 0.883831782 | 0.656091485 | 1.693482061 | 0.733860783 | 2.87311408 | 0.690666886 |
| 15 | N-Methylproline | Positive Hilic | 1.434443191 | 0.356711878 | 2.093768751 | 0.288915143 | 1.250040637 | 0.208222283 |
| 16 | N-Methylserine | Positive Hilic | 0.630955667 | 0.050240636 | 0.51163564 | 0.019520417 | 21.3565562 | 0.250600044 |
| 17 | Octopine | Positive Hilic | 5.287217325 | 0.197774148 | 12.78205406 | 0.172678835 | 0.738545396 | 0.019932737 |
| 18 | Proline–hydroxyproline | Positive Hilic | 1.699381081 | 0.015190354 | 2.008944192 | 0.033992985 | 1.515058751 | 0.174178233 |
| 19 | Propionylcarnitine | Positive Hilic | 1.178580994 | 0.131295737 | 0.804876763 | 0.7130271 | 11.61778227 | 0.530650712 |
| 20 | SDMA | Positive Hilic | 2.317179658 | 0.429051246 | 3.662975851 | 0.304548183 | 0.005772778 | 0.300783066 |
| 21 | Taurine | Positive Hilic | 1.152807858 | 0.378443043 | 1.135080404 | 0.532004759 | 0.724821458 | 0.939454594 |
| 22 | Uridine-5-diphosphoacetylglucosamine | Positive Hilic | 0.236797735 | 0.285847398 | 0.320233968 | 0.203784286 | 332.6518156 | 0.70071203 |
| 23 | Beta-alanine | Positive Hilic | 2.343509429 | 0.009954229 | 2.496581273 | 0.056740483 | 0.100688392 | 0.820501657 |
| 24 | Glycine | Positive Hilic | 0.46210005 | 0.16294956 | 0.487130168 | 0.056597298 | 2.098289127 | 0.766787981 |
| 25 | Palmitate | Positive Hilic | 0.614986594 | 0.464993079 | 1.090262794 | 0.880114504 | 0.002434426 | 0.600636379 |
| 26 | Cholate | Positive Hilic | 1.098018515 | 0.157506917 | 1.094544916 | 0.364194308 | 13.14097994 | 0.977100429 |
| 27 | Glycerophosphocholine | Positive Hilic | 0.410966821 | 0.028671262 | 0.361175125 | 0.173575736 | 9.097481347 | 0.726250188 |
| 28 | Glutamate | Positive Hilic | 1.221537042 | 0.207605635 | 1.025410416 | 0.690595173 | 2.45540182 | 0.176857341 |
| 29 | Leucine | Positive Hilic | 1.444908173 | 0.176917283 | 1.728757289 | 0.275269829 | 10.29942336 | 0.483717136 |
| 30 | 4-Quinolinecarboxylic acid | Negative Hilic | 1.418438368 | 0.513853945 | 2.159369267 | 0.218075199 | 0.103449101 | 0.086485699 |
| 31 | Aspartic acid | Negative Hilic | 0.482476444 | 0.065825708 | 0.602504147 | 0.113049977 | 0.127367313 | 0.516568913 |
| 32 | Glutamic acid | Negative Hilic | 1.046514892 | 0.780023849 | 0.827811878 | 0.242537341 | 0.150792733 | 0.415386587 |
| 33 | Ornithine | Negative Hilic | 1.850187949 | 0.391006337 | 5.755406751 | 0.145940321 | 0.078217893 | 0.165848257 |
| 34 | Pyroglutamic acid | Negative Hilic | 1.367259238 | 0.093669826 | 1.891645605 | 0.043315005 | 2.369792082 | 0.366558893 |
| 35 | Valine | Negative Hilic | 1.753550671 | 0.174813102 | 2.384865691 | 0.113296857 | 61.62403609 | 0.040640995 |
| 36 | Phenylalanine | Negative Hilic | 0.935194029 | 0.86993207 | 0.409014557 | 0.042444283 | 0.791986697 | 0.090863383 |
| 37 | Tryptophan | Negative Hilic | 0.371330134 | 0.081424087 | 0.192753035 | 0.042524748 | 0.01204981 | 0.032135949 |
| 38 | 4-Hydroxybenzaldehyde | Positive RP | 0.038566578 | 0.338542448 | 0.08710944 | 0.030486282 | 2.748006594 | 0.55634682 |
| 39 | 2-Quinolinecarboxylic acid | Positive RP | 1.284244421 | 0.178306336 | 1.296689552 | 0.039303244 | 1.799999525 | 0.963141641 |
| 40 | 4-Guanidinobutanoate | Positive RP | 0.542825386 | 0.391616229 | 1.237852105 | 0.544874186 | 1.46736347 | 0.207158492 |
| 41 | 4-Pyridoxate | Positive RP | 0.906943765 | 0.438937202 | 0.87509599 | 0.296192773 | 0.067630149 | 0.857936761 |
| 42 | Creatinine | Positive RP | 1.253400463 | 0.16561792 | 1.26221635 | 0.080361469 | 2.527037031 | 0.902938632 |
| 43 | Deoxycarnitine | Positive RP | 1.26442431 | 0.070202975 | 2.712227268 | 0.414190457 | 0.165367546 | 0.531546157 |
| 44 | d-pantothenic acid | Positive RP | 2.54453839 | 0.010420678 | 1.630641604 | 0.003710638 | 1.16289204 | 0.095092403 |
| 45 | Guanosine | Positive RP | 1.145849982 | 0.355689984 | 1.129363317 | 0.50751644 | 5.768618975 | 0.89840141 |
| 46 | Hippurate | Positive RP | 1.127259102 | 0.136289877 | 1.406303815 | 0.016533704 | 0.219676668 | 0.131795401 |
| 47 | Hypoxanthine | Positive RP | 1.359667365 | 0.457112959 | 1.608746416 | 0.099129029 | 7.05463603 | 0.809897126 |
| 48 | Carnitine | Positive RP | 1.90728994 | 0.143435009 | 1.564029523 | 0.172039547 | 0.018010572 | 0.589277748 |
| 49 | Proline | Positive RP | 1.1825476 | 0.10029429 | 1.364221698 | 0.019455593 | 1.09422421 | 0.087632586 |
| 50 | N-Acetyl-d-Tryptophan | Positive RP | 0.513159962 | 0.129235482 | 0.496551741 | 0.022394724 | 0.019329765 | 0.799179294 |
| 51 | Nicotinamide | Positive RP | 1.854686932 | 0.213557125 | 3.014205827 | 0.199623561 | 294.7130137 | 0.171017445 |
| 52 | Phenethylamine | Positive RP | 0.947278292 | 0.830253378 | 1.539430825 | 0.158597412 | 0.158329201 | 0.080856795 |
| 53 | Pipecolate | Positive RP | 1.25016381 | 0.293213253 | 1.427971372 | 0.207201663 | 1.071570022 | 0.09885495 |
| 54 | Pyridoxal | Positive RP | 1.217768919 | 0.031096573 | 1.27808282 | 0.111845597 | 0.112332187 | 0.515255364 |
| 55 | Pyridoxine | Positive RP | 1.087248254 | 0.317809478 | 1.096173651 | 0.700340773 | 4.820930313 | 0.965070128 |
| 56 | Cytidine | Positive RP | 2.322579759 | 0.180942623 | 2.507571618 | 0.258966415 | 29.54734542 | 0.869145933 |
| 57 | Citrulline | Positive RP | 1.24280421 | 0.682704165 | 0.603931311 | 0.531030346 | 0.000524472 | 0.53161591 |
| 58 | Inosine | Positive RP | 1.899386369 | 0.182257102 | 1.390470066 | 0.310097325 | 50.39782881 | 0.198914094 |
| 59 | Cytosine | Positive RP | 2.198331093 | 0.138106621 | 2.197735973 | 0.21587362 | 0.096448908 | 0.999444736 |
| 60 | Uracil | Positive RP | 0.944308633 | 0.227098922 | 1.026581624 | 0.238988701 | 0.184148495 | 0.131452792 |
| 61 | Guanine | Positive RP | 1.262679439 | 0.538371956 | 1.28191787 | 0.421569109 | 97.45431759 | 0.983003715 |
| 62 | Folate | Positive RP | 1.330385823 | 0.627752558 | 0.447595415 | 0.203196636 | 0.015697847 | 0.219888549 |
| 63 | Betaine | Positive RP | 2.045353528 | 0.181453617 | 2.995576467 | 0.1168387 | 2.012165194 | 0.007590226 |
| 64 | Pyroglutamate | Positive RP | 0.755668365 | 0.123082312 | 0.807394884 | 0.226135842 | 1566.855327 | 0.704018417 |
| 65 | Adenosine | Positive RP | 1.371651672 | 0.378510887 | 0.894426306 | 0.530775604 | 41.39432776 | 0.044975022 |
| 66 | 2-Hydroxyphenylacetate | Positive RP | 1.100243522 | 0.374777295 | 1.16996763 | 0.295039165 | 0.890312864 | 0.493264835 |
| 67 | Lipoamide | Positive RP | 1.23055559 | 0.104304526 | 1.21925258 | 0.072138847 | 0.442513586 | 0.929302085 |
| 68 | Suberate | Positive RP | 0.959311235 | 0.696818973 | 0.964122724 | 0.863292691 | 5.735613668 | 0.985533593 |
| 69 | 4-Methylcatechol | Positive RP | 0.816546234 | 0.152010857 | 0.621372018 | 0.037113323 | 0.807864486 | 0.0491021 |
| 70 | Uridine | Negative RP | 1.337277003 | 0.490020692 | 1.13709077 | 0.724444642 | 5.622283342 | 0.382538807 |
| 71 | 4-Acetamidobutanoate | Negative RP | 4.059047216 | 0.120056303 | 5.167043856 | 0.096549799 | 0.000822875 | 0.158797722 |
Italics values represent metabolites with significant values p < 0.05.