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. 2020 Jul 16;11(7):536. doi: 10.1038/s41419-020-02743-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The protocol for the noise exposure (NE) experiments is illustrated. b Auditory brainstem response (ABR) thresholds (dB sound pressure level [SPL]) at click (CK), 8, 16, 24, and 32 kHz in WT and DIA1^TG/TG (TG) mice were measured at the age of 4 weeks (4 W, just before and after NE at day 0) and 8 weeks (8 W, post-NE at day 28). Note the NE-induced a temporary threshold shift (TTS) both in WT and TG mice (n = 14). c The percentages of remaining inner hair cells (IHCs) and outer hair cells (OHCs) at each cochlear turn (Ap: apical, Md: middle, and Bs: basal turn) in WT and TG mice at day 28 after NE. There was no significant difference between WT (n = 8) and TG mice (n = 7). d Distortion product otoacoustic emission (DPOAE) measurement with pure-tone bursts at 4, 6, 8, 10, 12, 16, 18, 20, and 24 kHz at day 28 after NE. There was no significant difference between WT and TG mice (n = 8). e At day 28 after NE experiments (at 8-weeks-old), three turns of the cochlea (Ap, Md, and Bs) were obtained, and immunostained using a CtBP2 antibody. Each image shows confocal microscopic images obtained from the center of each cochlear turn, and the number of CtBP2-positive puncta in each image is indicated. In the absence of NE, there was no significant difference in the number of CtBP2-positive puncta in IHC at each cochlear turn, between WT and TG mice. NE-induced significant decreases in the number of puncta of IHCs at Bs in TG mice compared to WT mice. Scale bar: 20 μm. f The cochleogram shows the number of CtBP2-positive puncta per IHC in each percentage distance from the apex of the cochlea at 8 weeks, with or without NE. In the post-NE group, a significant decrease at the distance of 80% from apex was observed in TG mice compared with WT mice (n = 7). **p = 0.0036 by two-way ANOVA with Bonferroni post-hoc testing. g The wave I amplitudes with 90 dB SPL at CK, 16 and 32 kHz at day 28 after NE in WT (n = 20) and TG (n = 24) mice were graphed. There were no significant differences between WT and TG mice.