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. 2020 Jul 17;7(4):551–557. doi: 10.1016/j.gendis.2020.07.006

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© 2020 Chongqing Medical University. Production and hosting by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Detection of neutralizing antibodies in sera against SARS-CoV-2 pseudovirus infection. (A) Inhibition of SARS-CoV-2 pseudovirus entry by ACE2-Ig. S-C19del or VSV-G pseudovirions were preincubated with ACE2-Ig and added to 293T-ACE2 cells. (B) Sera from convalescent COVID-19 patients neutralized the SARS-CoV-2 pseudovirus. Serum sample from healthy individual was tested as a negative control. RLU were detected at 72 h post-pseudovirus inoculation. The data are presented as the mean percentages of inhibition ± SDs of duplicate wells.