Table 3. List of fish haplotypes with the GenBank numbers identified from the eDNA metabarcoding study of the four rivers.

No.	Family	Haplotype ID	Haplotypes	Identity (%)	Korean haplotype	Chinese haplotype	Japanese haplotype	Others
1	Gobiidae	SJ3	Acanthogobius hasta	100	KM030428	KM891736	–
2	Gobiidae	TH3	Acanthogobius lactipes	100	KM030431	–	LC385140
3	Cyprinidae	SJ1	Acheilognathus intermedia	99	EF483933	–	–
4	Cyprinidae	HS1	Acheilognathus macropterus	99	EF483935	KJ499466	LC092100
5	Cyprinidae	SJ1	Acheilognathus majusculus	99	–	–	LC006056
6	Cyprinidae	SJ2	Acheilognathus rhombeus	99	KT601094	–	LC146100
7	Cyprinidae	SJ1	Acheilognathus sp. (unidentified)	95			LC006056
8	Anguillidae	TH4	Anguilla japonica	100	HQ185628	MH050933	LC193417
9	Cyprinidae	HS1	Carassius auratus	100	–	KX505165
10	Cyprinidae	TH2	Carassius auratus	100				Turkey KM657132
11	Cyprinidae	TH3	Carassius auratus	99		AY771781	LC193299
12	Cyprinidae	SJ2	Carassius auratus	99	–	AY771781	LC193299
13	Cyprinidae	TH3	Carassius cuvieri	100	–	–	AP011237
14	Cyprinidae	SJ3	Carassius cuvieri	100			AP011237
15	Channidae	TH1	Channa argus	100	–	MG751766	AB972107
16	Cobitidae	TH1	Cobitis sp.	97	EU670794	–	LC146139
17	Cobitidae	TH1	Cobitis sp.	97	EU670794	–	LC146139
18	Cobitidae	SJ2	Cobitis tetralineata	100	EU670794	–	LC146139
19	Cobitidae	SJ1	Cobitis tetralineata	99	EU670794	–	LC146139
20	Cyprinidae	SJ1	Coreoleuciscus sp. (unidentified)	96	JN831358	–	AP011258
21	Cyprinidae	SJ1	Coreoleuciscus splendidus	100	JN831358	–	AP011258
22	Sinipercidae	HS3	Coreoperca herzi	100	KR075132	–	–
23	Sinipercidae	SJ1	Coreoperca sp.	97	KR075132	–	–
24	Cyprinidae	ND4	Cyprinus carpio	100	–	KX710076	AP017363
25	Cyprinidae	HS2	Cyprinus carpio	100	–	KX710076	AP017363
26	Cyprinidae	ND3	Cyprinus carpio	99	–	KX710076	AP017363
27	Cyprinidae	TH2	Cyprinus megalophthalmus	100	–	KR869143	–
28	Gobiidae	SJ3	Favonigobius gymnauchen	100	–	–	LC385206
29	Gobiidae	HS1	Gymnogobius breunigii	99	KM030451	–	–
30	Gobiidae	HS1	Gymnogobius sp.	98	KM030451	–	–
31	Gobiidae	TH3	Gymnogobius sp.	98	KM030451	–	–
32	Cyprinidae	SJ1	Hemibarbus labeo	100	DQ347953	KP064328	LC049898
33	Cyprinidae	ND2	Hemibarbus maculatus	99	–	NC018534
34	Cyprinidae	SJ1	Hemibarbus sp.	97	DQ347953	KP064328	LC049898
35	Cyprinidae	SJ2	Hemibarbus sp.	97	DQ347953	KP064328	LC049898
36	Cyprinidae	TH4	Hemibarbus sp. (unidentified)	95	DQ347953	KP064328	LC049898
37	Cyprinidae	ND1	Hemiculter leucisculus	100	–	–	LC340359
38	Cobitidae	SJ1	Iksookimia longicorpa	100	KM676413	–	LC146135
39	Cobitidae	HS1	Iksookimia yongdokensis	100	EU670800	–	–
40	Cobitidae	TH2	Iksookimia yongdokensis	99	EU670800	–	–
41	Pleuronectidae	SJ3	Kareius bicoloratus	100	–	–	AP002951
42	Clupeidae	TH3	Konosirus punctatus	100	–	KC477844	LC020951	Taiwan AP011612
43	Clupeidae	ND3	Konosirus punctatus	99	–	KC477844	LC020951	Taiwan AP011612
44	Centrarchidae	TH4	Lepomis macrochirus	100	–	JN389795	AP005993	USA KP013118
45	Amblycipitidae	SJ1	Liobagrus sp.	97	KR075136	KX096605	AP012015
46	Cyprinidae	SJ2	Microphysogobio koreensis	100	FJ515920	–	–
47	Cyprinidae	SJ1	Microphysogobio yaluensis	99	KR075133	–	AP012073
48	Centrarchidae	ND1	Micropterus salmoides	100	–	HQ391896	LC069536	USA DQ536425
49	Centrarchidae	HS1	Micropterus salmoides	99	–	HQ391896	LC069536	USA DQ536425
50	Cobitidae	SJ1	Misgurnus anguillicaudatus	100	–	KC762740	–
51	Cobitidae	TH1	Misgurnus anguillicaudatus	99	–	KC762740	–
52	Cobitidae	SJ2	Misgurnus anguillicaudatus	99	EU670804	–	–
53	Cobitidae	HS1	Misgurnus anguillicaudatus	99	–	–	LC385093
54	Cobitidae	HS1	Misgurnus bipartitus	100	–	KF562047	LC091592
55	Cobitidae	TH3	Misgurnus mizolepis	100	AP017654	–	–
56	Cobitidae	HS3	Misgurnus mizolepis	99	AP017654	–	–
57	Mugilidae	HS1	Mugil cephalus	100	–	KF374974	LC278014
58	Gobiidae	TH3	Mugilogobius abei	100	KM030465	–	LC421743	Taiwan KF128984
59	Cyprinidae	TH1	Nipponocypris koreanus	100	–	KJ427719	–
60	Cyprinidae	HS1	Nipponocypris temminckii	100	–	–	AP012116
61	Cobitidae	TH1	Niwaella multifasciata	100	EU670807	–	LC146133
62	Cobitidae	HS1	Niwaella sp. (unidentified)	96	EU670807	–	LC146133
63	Odontobutidae	SJ1	Odontobutis interrupta	100	KR364945	–	–
64	Odontobutidae	HS1	Odontobutis platycephala	100	KM030426	–	–
65	Odontobutidae	SJ2	Odontobutis platycephala	99	KM030426
66	Cyprinidae	HS1	Opsariichthys sp. (unidentified)	96	–	–	AB218897
67	Cyprinidae	TH3	Opsariichthys uncirostris	99	–	–	AB218897
68	Cobitidae	TH4	Paramisgurnus dabryanus	100	–	KM186182	LC146125
69	Cobitidae	HS1	Paramisgurnus dabryanus	100	–	KJ699181	LC146125
70	Cyprinidae	SJ2	Phoxinus oxycephalus	99	MK208924	–	AB626852
71	Cyprinidae	SJ3	Phoxinus oxycephalus	99	MK208924	–	AB626852
72	Cyprinidae	TH3	Phoxinus semotilus	100	KT748874	–	–
73	Mugilidae	TH3	Planiliza affinis	100	–	KM925142	LC277843
74	Mugilidae	SJ2	Planiliza haematocheila	100	–	KJ622047	LC021099
75	Mugilidae	HS4	Planiliza haematocheila	100	–	KJ622047	LC021099
76	Bagridae	SJ1	Pseudobagrus koreanus	100	KT601095	–	–
77	Bagridae	ND1	Pseudobagrus ussuriensis	100	–	KC188782	–
78	Bagridae	ND2	Pseudobagrus ussuriensis	99	–	KC188782	–
79	Cyprinidae	ND2	Pseudogobio esocinus	100	–	–	LC340042
80	Cyprinidae	ND1	Pseudogobio esocinus	99	–	–	LC340042
81	Cyprinidae	ND3	Pseudogobio vaillanti	100	–	KU314695	LC146041
82	Cyprinidae	SJ2	Pseudogobio vaillanti	99	–	KU314695	LC146041
83	Gobiidae	TH3	Pseudogobius masago	100	KM030467	–	LC049791
84	Cyprinidae	TH1	Pungtungia herzi	99	KF006339	–	AB239598
85	Cyprinidae	SJ1	Pungtungia sp.	97	KF006339	–	AB239598
86	Cyprinidae	TH1	Pungtungia sp. (unidentified)	96	KF006339	–	AB239598
87	Gobiidae	HS1	Rhinogobius brunneus	100	KT601096	–
88	Gobiidae	ND2	Rhinogobius brunneus	100			LC049760
89	Gobiidae	ND1	Rhinogobius giurinus	100	KM030475	KP892753	LC049748
90	Cyprinidae	SJ2	Rhodeus suigensis	100	EF483934	–	–
91	Cyprinidae	SJ1	Rhodeus uyekii	100	EF483937	–	–
92	Cyprinidae	HS1	Rhynchocypris lagowskii	99	–	KJ641843	–
93	Cyprinidae	TH3	Rhynchocypris lagowskii	99		KJ641843
94	Cyprinidae	TH4	Rhynchocypris lagowskii	99		KJ641843
95	Cyprinidae	SJ2	Rhynchocypris oxycephalus	99	–	–	LC193377
96	Cyprinidae	SJ3	Rhynchocypris oxycephalus	99			LC193377
97	Cyprinidae	HS4	Rhynchocypris sp.	98			LC193377
98	Cyprinidae	HS2	Sarcocheilichthys soldatovi	100	–	–	LC146036
99	Cyprinidae	HS2	Sarcocheilichthys sp.	97	KU301744	–	AP012067
100	Cyprinidae	ND3	Sarcocheilichthys sp.	97	KU301744	–	AP012067
101	Cyprinidae	SJ2	Sarcocheilichthys variegatus	100	KU301744	–	AP012067
102	Siluridae	ND1	Silurus asotus	100	–	JX087351	NC015806
103	Siluridae	TH1	Silurus microdorsalis	99	KT350610	–	–
104	Siluridae	SJ1	Silurus sp. (unidentified)	96	KT350610
105	Sinipercidae	SJ1	Siniperca scherzeri	100	–	MF966985	–	Taiwan AP014527
106	Cyprinidae	SJ2	Squalidus chankaensis	100	KT948082	–	–
107	Cyprinidae	HS3	Squalidus japonicus	100			LC277782
108	Cyprinidae	SJ3	Squalidus japonicus	99			LC277782
109	Cyprinidae	TH3	Squalidus japonicus coreanus	100	KR075134	–
110	Cyprinidae	HS1	Squalidus multimaculatus	100	KX495606	–	–
111	Bagridae	SJ1	Tachysurus fulvidraco	100	–	KU133295	LC193372
112	Bagridae	ND2	Tachysurus nitidus	100	–	KC822643	–
113	Cyprinidae	SJ1	Tanakia signifer	99	EF483930	–	–
114	Cyprinidae	SJ2	Tanakia somjinensis	99	FJ515921	–	–
115	Cyprinidae	SJ1	Tanakia sp.(unidentified)	96	FJ515921
116	Cyprinidae	TH2	Tribolodon hakonensis	100	–	–	AB626855
117	Cyprinidae	SJ3	Tribolodon hakonensis	99	–	–	AB626855
118	Gobiidae	TH4	Tridentiger obscurus	100	KT601092	MF663787	LC193168
119	Gobiidae	SJ2	Tridentiger radiatus	99	–	EU047755	–
120	Gobiidae	ND2	Tridentiger radiatus	99
121	Gobiidae	SJ3	Tridentiger trigonocephalus	100	KM030481
122	Gobiidae	HS4	Tridentiger trigonocephalus	100		KT282115	LC385175
123	Cyprinidae	SJ1	Zacco platypus	100	–		LC277796
124	Cyprinidae	HS1	Zacco platypus	99		KF683339
125	Cyprinidae	TH1	Zacco sp.	97		KF683339