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. 2020 Jul 10;11:1021. doi: 10.3389/fpls.2020.01021

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Copyright © 2020 Seifbarghi, Borhan, Wei, Ma, Coutu, Bekkaoui and Hegedus

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Necrosis-inducing activity of Sclerotinia sclerotiorum necrosis-inducing effectors using Agrobacterium tumefaciens-mediated transient expression in Nicotiana benthamiana. Leaves were infiltrated with A. tumefaciens harboring various constructs. Infiltrated zones on each leaf show constructs expressing S. sclerotiorum effectors with a signal peptide (SP), constructs expressing S. sclerotiorum effectors without a signal peptide (ΔSP), a construct expressing the empty vector as negative control (N), and a construct expressing the Nep1-like protein (PiNPP1.1) from Phytophthora infestans as positive control (P).