Figure 5.

The effects of cysteine residue mutation and derived peptides from Sclerotinia sclerotiorum SsNE2 on the necrotizing activity. (A) SsNE2 cysteine residues at positions of C³⁸, C⁴⁵, C⁶⁴, and C⁸⁶ were either individually or simultaneously replaced by alanine and constructs infiltrated into N. benthamiana leaves using A. tumefaciens. Wild type SsNE2 used as a positive control and A. tumefaciens strains carrying an empty vector as a negative control (N). (B) Schematic representation of peptides derived from SsNE2 (left hand panels denoted as M1, M2, M3, M1+M2, and M2+M3) that were infiltrated into N. benthamiana leaves (right hand panels).