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. 2020 Jul 10;10:1102. doi: 10.3389/fonc.2020.01102

Figure 3.

Figure 3

STAT3/HSP90 interplay sustains mutp53 expression level. U373 cell line, cultured with 100 μM AG490 (A) or STAT3 silenced (B) for 48 h, was analyzed by western blot for HSP90 protein expression. Lamin B and β Actin were used as loading control. In (C), U373 cells pre-treated or not with 5 nM of bortezomib (BZ) and then treated with 100 μM AG490 for 48 h were analyzed by western blot for the expression of p53 and MVK. In (D–F) U373 (D,F) and Panc1 (E) cells were cultured with 100 nM geldanamycin (GELDA) and the expression of mutp53, STAT3, and MVK (F) was evaluated by western blot. In (G), the expression of HSP90 in U373 cells cultured for 24 h with 50 μM lovastatin (LOVA) was analyzed by western blot. β Actin was used as loading control. One representative experiment out of 3 is shown. The histograms represent the mean plus S.D. of the densitometric analysis of the ratio of specific band and control of 3 different experiments. *P < 0.05.