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. 2020 Jul 10;10:1102. doi: 10.3389/fonc.2020.01102

Figure 5.

Figure 5

STAT3 inhibition in wtp53 U87 cells reduces cell survival and increase p53 and p21 expression and inhibits the mevalonate pathway. U87 cells were cultured in the absence or in the presence of 100 μM AG490 for 48 h, and cell survival and STAT3, p53, p21, HSP90, and MVK expression were analyzed, respectively, by trypan blue exclusion assay (A) and by western blot (B–F). The histograms (A) represent the mean plus S.D. of more than 3 experiments *P < 0.05; in western blot (B–F) β Actin was used as loading control. One representative experiment out of 3 is shown. The histograms represent the mean plus S.D. of the densitometric analysis of the ratio of specific band and control of 3 different experiments. In (G), FACS analysis of ROS production, by U373, Panc1, and U87 treated or not with 100 μM AG490, measured by DCFDA staining. The mean of fluorescence intensity is indicated. Solid gray peaks represent the controls. One representative experiment out of 3 is shown.