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. 2020 Jun 17;48(13):7520–7531. doi: 10.1093/nar/gkaa513

Figure 3.

Figure 3.

OAS/RNase L pathway activation in A549 cells by the short dsRNAs correlates with their ability to activate OAS1 in vitro. (A) Bioanalyzer analysis of rRNA integrity in A549 cells following transfection with the indicated dsRNAs (18 bp dsRNAs with 3′-ssPy motifs appended as indicated and an 18 bp Scramble dsRNA). Mock transfection and transfection with poly(rI:rC) dsRNA serve as negative and positive controls, respectively. OAS1/RNase L pathway activation is indicated by 28S and 18S rRNA degradation (arrows). Quantification of the 28S rRNA cleavage product (solid arrow) is given in Table 1. (B) As for panel A but using A549 RNase L KO cells (6). In both panels, a representative analysis with technical duplicates for each RNA is shown for one of at least two independent experiments which showed essentially identical results.