Table 1.
Summary of OAS1 activation by short dsRNAs in vitro and A549 cells
| Kinetic analysisb | ||||
|---|---|---|---|---|
| 3′-ssPy location | Initial rate (nmol PPi/ min)a | K app (μM) | V max (nmol PPi/ min) | Relative 28S rRNA cleavagec |
| None | 1.4 ± 0.1 | 10.3 ± 1.0 | 36.0 ± 2.5 | 1.0 |
| Top | 3.5 ± 0.3 | 4.0 ± 0.3 | 36.5 ± 1.4 | 1.7 ± 0.5 |
| Bottom | 1.6 ± 0.2 | 6.5 ± 0.6 | 22.8 ± 1.5 | 0.6 ± 0.2 |
| Both ends | 4.0 ± 0.2 | ND | ND | 2.7 ± 0.2 |
| Initial rate (nmol PPi/ min)a | ||||
| Base Pair Move | Scramble (Consensus) | Consensus Strand Swap | ||
| None | 2.0 ± 0.1 | 0.74 ± 0.05 | 0.68 ± 0.06 | |
| Top | 2.4 ± 0.2 | 1.3 ± 0.1 | 1.0 ± 0.1 | |
| Bottom | 2.7 ± 0.3 | 0.80 ± 0.04 | 0.64 ± 0.04 | |
| Both ends | 2.8 ± 0.3 | 1.2 ± 0.1 | 0.95 ± 0.04 | |
aInitial rates determined by linear regression analysis on the 0–10 min time points for replicate time course experiments performed at a single dsRNA concentration (300 nM dsRNA), as shown in Figures 2, 5, and 6.
bKinetic parameters (Kapp and Vmax) were determined from fits to data shown in Figure 4. ND, Not determined.
cValues from quantification of three independent experiments in wild-type A549 cells, exemplified in Figure 3.