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. 2020 Jun 11;48(13):7079–7098. doi: 10.1093/nar/gkaa485

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — Comparison of mRNA levels (RNA-Seq) and ribosome profiling levels for RP genes. (A–G) Scatter plot of matched mRNA (measured by RNA-seq) and ribosome profiling levels (showing level of transcripts being translated) for RP genes in various tissues/cell-types. Median value is shown for tissue/cell-type with multiple samples. The axes are in log₂ scale. RPF = ribosome protected fragment, hES = human embryonic stem cells grown in a feeder free protocol that maintains pluripotency, NDx = human embryonic stem cell grown for x days in a neural conversion protocol that induces cell differentiation. (H) Spearman Rank Correlation of mRNA and ribosome profiling levels of RP genes for paired samples shows highly consistent covariation and highly significant correlation (P < 10⁻⁴) of mRNA and ribosome profiling levels for RP genes in each individual sample.