Table 1.
Data collection and refinement statistics of APE1:DNA co-complexes
| Y269A product (AP-DNA) | Y269A substrate (AP-DNA) | |
|---|---|---|
| Data collection | ||
| Space group | P 21 21 21 | P 21 21 21 |
| Cell dimensions | ||
| a, b, c (Å) | 45.38,150.23,156.45 | 45.66,148.32,153.24 |
| α, β, γ (°) | 90.00,90.00,90.00 | 90.00,90.00,90.00 |
| Resolution (Å) | 50.00–2.20 | 48.29–2.77 |
| R meas (%) | 0.073 (0.440) | 0.261 (2.670) |
| I/σI | 20.6 (2.3) | 7.4 (0.8) |
| cc1/2 | (0.792) | (0.395) |
| Completeness (%) | 99.5 (96.9) | 100.0 (100.0) |
| Redundancy | 5.9 (3.5) | 6.8 (6.8) |
| Refinement | ||
| Resolution (Å) | 43.58–2.20 | 48.29–2.77 |
| No. reflections | 91 210 | 27 446 |
| R work/ R free | 18.55/22.73 | 21.85/27.30 |
| No. atoms | ||
| Protein | 4379 | 4382 |
| DNA | 1669 | 1669 |
| Water | 363 | 39 |
| B-factors (Å2) | ||
| Protein | 28.73 | 46.81 |
| DNA | 62.14 | 75.36 |
| Water | 33.86 | 40.54 |
| R.m.s deviations | ||
| Bond length (Å) | 0.008 | 0.017 |
| Bond angles (°) | 1.018 | 1.432 |
| PDB ID | 6W4I | 6W4T |
Each structure was obtained from a single crystal. Values in parentheses are for highest-resolution shell.