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Design of an in vitro selection protocol for a CuAAC-catalyzing DNAzyme. The ‘N40’ region shown in red indicates the location of the randomized stretch of DNA sequence present within each individual DNA molecule (∼1014 total such molecules within the starting selection ‘library’). The blue arrow (right) shows the direction of electrophoretic migration of DNA in a denaturing polyacrylamide gel (PAGE).
