Skip to main content
. 2020 Jun 10;48(13):7356–7370. doi: 10.1093/nar/gkaa502

Figure 2.

Figure 2.

In cis catalysis by 5′-hexynyl-CLICK-17 DNA (≡-CLICK-17). Panels A and B: 5′-hexynyl-CLICK-17 DNA (≡-CLICK-17) catalyzes CuAAC in cis in R buffer (A); by contrast, 5′-hexynyl-PERMUT-17 DNA (≡-PERMUT-17) does not catalyze the reaction (B). Reaction conditions were: 2 μM ≡-CLICK-17 (or ≡-PERMUT-17) and 2.5 mM azide-biotin in R buffer, to which 100 μM CuSO4 (the default concentration, unless indicated), and 2.5 mM sodium ascorbate were added. Reactions proceeded at 22°C for 1 h except in the copper titration lanes (where they proceeded for 30 min).