Table 2.
Short double-stranded region of the terminator hairpin of malM-3′ RNA is sufficient for binding by the ProQ NTD
K d [nM] | |
---|---|
32P-RNA | NTD |
malM-3′ | 12 ± 4.8a |
malM-3′-bulge | 9.0 ± 2.5 |
malM-3′-AA | 6.6 ± 1.4 |
malM-3′-UU | 8.7 ± 2.1 |
malM-3′-4bp | 8.8 ± 2.4 |
malM-3′-3bp | 8.7 ± 2.4 |
malM-3′-2bp | 6.4 ± 2 |
malM-3′-1bp | >1000 |
The Kd values were obtained by fitting the data using the quadratic equation. The average Kd values with standard deviations were calculated from three or four independent experiments, except for malM-3′ (eleven replicates).
aData from Table 1.