Table 3.

The length of single-stranded sequence following the Rho-independent terminators affects the binding of malM-3′ RNA to the N-terminal domain of ProQ

	K d [nM]
32P-RNA	NTD
malM-3′-9U	5.1 ± 2.9
malM-3′ (7U, wt)	12 ± 4.8a
malM-3′-6U	7.8 ± 1.2
malM-3′-5U	9.7 ± 1.5
malM-3′-4U	27 ± 11
malM-3′-3U	>1000
malM-3′-2U	n. d.
malM-3′-1U	n. d.

The K_d values were obtained by fitting the data to the quadratic equation. The average K_d values with standard deviations were calculated from three to five independent experiments, except for malM-3′ (eleven replicates).

^aData from Table 1.

n. d.: the binding was essentially undetectable up to 1 μM concentration of the NTD.