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. 2020 Jun 8;48(13):7182–7196. doi: 10.1093/nar/gkaa482

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Comparison of gene expression in wild type and Mst KO day 4 EBs and directed mesoderm differentiation. (A) Heatmap of RNA-seq expression data of Mst KO and WT day 4 EBs. Representative differentially expressed genes (DEGs) are indicated. (B) GO enrichment of upregulated and downregulated genes in day 4 Mst KO versus WT EBs. (C) Gene Set Enrichment Analysis (GSEA) of differentially expressed genes between Mst KO and WT day 4 EBs among genes associated endoderm development, mesoderm formation, cardiocyte differentiation and embryonic placenta development. (D) Morphology of WT and Mst KO ESC suspension culture after 24 h growth in mesoderm induced medium. Scale = 200 μm. (E) Real-time qPCR result of mRNA level of nascent mesodermal genes (Mesp1) and mesendoderm marker (Eomes, T) in WT and Mst KO samples at ESC stage and 24h after mesoderm directed induction. All data are presented as the mean ± SD (n = 3). Gapdh was used as an internal control. Statistically significant differences are indicated (*P< 0.05; **P< 0.01; ***P< 0.001).