Table 2.
Data collection and refinement statistics
| Data collection | |
| Detector type/source | Rayonix CCD/APS LS-CAT |
| Wavelength (Å) | 0.97872 |
| Space group | P6522 |
| Cell parameters | a = b = 118.28 Å, c = 261.55 Å |
| Number of data sets | 5 |
| Resolution range (Å)a | 39.18–3.28 (3.46–3.28) |
| Total number of observations | 720 160 (41 046) |
| Unique reflections | 15 332 (760) |
| Mean (I)/σ(I) | 18.2 (1.8) |
| Completeness (spherical) (%)b | 88.3 (30.7) |
| Completeness (ellipsoidal) (%) | 92.8 (43.8) |
| Multiplicity | 47 (54) |
| R merge (all I+ & I−) | 0.160 (4.129) |
| R merge (within I+/I−) | 0.176 (4.153) |
| R meas (all I+ & I−) | 0.162 (4.168) |
| R meas (within I+/I−) | 0.179 (4.227) |
| R pim (all I+ & I−) | 0.025 (0.561) |
| R pim (within I+/I−) | 0.36 (0.784) |
| CC(1/2) | 0.996 (0.598) |
| Refinement | |
| Number of reflections working/test | 14,553/785 |
| R (working set; %) | 26.37 (37.6) |
| R free (test set; %) | 27.93 (61.2) |
| Structure quality | |
| RNA atoms | 3174 |
| Other atoms | 227 |
| RMS deviations in bond lengths (Å) | 0.003 |
| RMS deviations in bond angles (°) | 0.807 |
| Average B factor (Å2) (RNA) | 137.4 |
| Average B factor (Å2) (all atoms) | 136.8 |
aNumbers in parenthesis correspond to the highest resolution shell.
bAnisotropic data were processed used the Staraniso server (36).