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. 2020 Jun 17;48(13):7041–7051. doi: 10.1093/nar/gkaa505

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — ¹⁹F NMR experiments for study B-Z transition in vitro. (A) Concept for the detection of B–Z transition by ¹⁹F NMR. Two ¹⁹F resonances of different chemical shifts are expected according to B-DNA and Z-DNA. (B) ¹⁹F NMR spectra of d(CGC^FGCG)₂ in 1 mM Na-PO₄ buffer (pH 7.0) and various NaCl concentrations. (C) ¹⁹F NMR spectra of d(C^FGCAC^FGCG)/d(CGCGTGCG) in 1 mM Na-PO₄ buffer (pH 7.0) and various NaCl concentrations. The ¹⁹F NMR spectra were recorded at 50 μM duplex concentration. Red and black spots indicated B-form and Z-form DNA, respectively.