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. 2020 Jun 19;48(13):7197–7217. doi: 10.1093/nar/gkaa287

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Simple terminal truncations. (A) Telomeric PstI fragment containing 2539TEL10 showing MoTeR sequences embedded in the telomere. (B) TRF segregation among progeny of a cross between 2539 (MoTeR⁺) and Guy11 (MoTeR⁻). The top panel shows the phosphorimage of a Southern hybridization analysis of PstI-digested DNAs probed with telomere repeats. Parental strain DNAs are loaded in the outermost lanes, with progeny DNAs in between. 2539TEL10.i is marked with a black arrowhead. A white arrowhead marks a novel TRF that corresponds to a TEL10 variant (ii). Bottom panel: the membrane used in A was stripped and re-hybridized with a MoTeR2 probe. (C) Organization of 2539TEL10.ii showing 5′-truncation of the terminal MoTeR2. (D) 106 bp of sequence surrounding the site of de novo telomere addition at the MoTeR2 truncation boundary. Telomere repeats are highlighted with bold text. A ‘T’ nucleotide that seeded the de novo telomere addition is underlined. Inferred rearrangement mechanism: i → ii) MoTeR truncation, de novo telomere formation.