Table 1.
Antibodies used in the study
| Antibodies | Company (Cat. No.) | Applications |
|---|---|---|
| DICER (rabbit) | Sigma (SAB4200087) | IF (1:100), WB (1:200) |
| DICER (rabbit) | Bethyl Laboratories, Inc. (A301-936A) | IP, ChIP |
| SUV39H2 (rabbit) | Abcam (EPR18495) | ChIP |
| SUV39H2 (rabbit) | Proteintech Group, Inc. (11338-1-AP) | IP, WB (1:500) |
| SETDB1 (rabbit) | Proteintech Group, Inc. (11231-1-AP) | IP, WB (1:500) |
| HP1β (mouse) | Millipore (MAB3448) | IF (1:25) |
| H3K9me3 (rabbit) | Millipore (07-442) | IF (1:100), ChIP |
| MILI/PIWIL2 (mouse) | Sigma (MABE363) | IF (1:300) |
| TDRD1 (rat) | R&D Systems (MAB6296) | IF (1:200) |
| DDX25 (goat) | Santa Cruz Biotechnology (sc-51271) | IF (1:100) |
| phospho-Histone H2A.X (Ser139) (mouse) | Millipore (05-636) | IF (1:500) |
| phospho RNA Polymerase II (S2) (rabbit) | Bethyl Laboratories, Inc. (A300-654A) | WB (1:500) |
| β-TUBULIN (rabbit) | Cell Signaling (2128S) | WB (1:1000) |
| GAPDH (mouse) | Hytest Ltd. (5G4-6C5) | WB (1:3000) |
| Cytochrome C (mouse) | BD Biosciences (556433) | IF (1:300) |
| DDX4 (rabbit) | Abcam (ab13840) | WB (1:500) |
| YY1 (rabbit) | Bethyl Laboratories, Inc. (A302-779A-M) | WB (1:500) |
| Histone H3 (rabbit) | Cell Signaling (4499S) | WB (1:300) |
| TRIM33 (rabbit) | Bethyl Laboratories, Inc. (A301-060A-M) | WB (1:500), IP, IF (1:50) |
| TRIM28 (rabbit) | Bethyl Laboratories, Inc. (A304-145A-M) | IP |
| TRIM28 (rabbit) | Bethyl Laboratories, Inc. (A300-274A-M) | WB (1:500), IF (1:100) |
| Negative control IgG (mouse) | Santa Cruz Biotechnology (sc-2025) | IP,ChIP |
| Negative control IgG (rabbit) | Neomarkers (NC100-P1) | IP, ChIP |
| Donkey anti-mouse IgG Alexa Fluor 488 conjugate | Invitrogen (A-21202) | IF (1:1000) |
| Donkey anti-mouse IgG Alexa Fluor 594 conjugate | Invitrogen (A-21203) | IF (1:1000) |
| Donkey anti-rabbit IgG, Alexa Fluor 488 conjugate | Invitrogen (A-21206) | IF (1:1000) |
| Donkey anti-rabbit IgG Alexa Fluor 594 conjugate | Invitrogen (A-21207) | IF (1:1000) |
| Donkey anti-rat IgG Alexa Fluor 488 conjugate | Invitrogen (A-21208) | IF (1:1000) |
| Anti-rabbit HRP-conjugated | Cell Signaling (7074S) | WB (1:1000) |
| Anti-rouse HRP-conjugated | Cell Signaling (7076S) | WB (1:1000) |
| Anti-rabbit light chain HRP-conjugated | Millipore (MAB201P) | WB (1:1000) |