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. 2020 Jun 15;48(13):7027–7040. doi: 10.1093/nar/gkaa504

Figure 1.

Figure 1.

Detection of 2′Ome of human miRNAs at the 3′-end. (A) LC−MS/MS chromatogram in MRM mode of the synthetic nucleosides (standard, std) and authentic nucleosides isolated from non-cancerous lung tissue (N) and NSCLC lung tissue (C). (B) Comparison of nucleoside methylation in the indicated small RNAs (18–24nt) from non-cancerous lung tissue (N) and NSCLC lung tissue (C). (C) Heatmap of 2′Ome at the 3′-end of human miRNAs in non-cancerous lung tissue (N) and NSCLC lung tissue (C) after oxidation procedure. N-oxid: non-cancerous lung tissue with oxidation. C-oxid: NSCLC lung tissue with oxidation. (D) Detection of 2′Ome of human miRNAs at the 3′-end by qRT-PCR after oxidation procedure. (E) Detection of 2′Ome of human miRNAs at the 3′-end by northern blotting after Oxid/β-elim procedure.