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. 2020 Jun 15;48(13):7027–7040. doi: 10.1093/nar/gkaa504

Figure 7.

Figure 7.

3′-Terminal 2′Ome of human miR-21-5p increases its association with AGO2 complex and inhibition on PDCD4 expression. (A) Depict of experimental strategy of assaying binding affinity of miR-21-5p or miR-21-5pCH3 with AGO2 complex using Cy3- or Cy5-labeled miRNAs. (B) Binding affinity of miR-21-5p and miR-21-5pCH3 with AGO2 complex detected by fluorescence intensity measurement. (C) Kinetics of binding of miR-21-5p and miR-21-5pCH3 with AGO2 detected by microscale thermophoresis. (D) Binding of miR-21-5p or miR-26-5p with AGO2 detected by the luciferase activity in A549 or A549KO cells transfected with miR-21-5p or miR-26-5p reporter plasmid. (E) PDCD4 and ULK1 protein level in A549 and A549KO cells at different time points after serum depletion. (F) Apoptosis of A549 and A549KO cells induced by serum depletion.