Figure 2.

Roquin recognizes wild type and stabilized UCP3 AREs in a CDE-like manner. (A and B) Overlay of ¹H-¹⁵N-HSQCs showing ROQ alone and in complex with UCP3 wild type CDE1 (A) and CDE2 (B). The bar plots below the spectral overlays show the combined CSPs of amide resonances along with the primary ROQ domain sequence. (C and D) The same as in panels (A) and (B), but with CDE1GG (C) and CDE2GG (D) SLs from the UCP3 3′-UTR. The insets consistently show an equivalent zoom-in from all overlays highlighting a part of the glycine region. Selective amide assignments are given for orientation. Negative bars indicate residues with missing assignments in at least one of the two spectra or prolines. See also Supplementary Figure S2.