Figure 5.

The ROQ domain locks the UCP3 CDE-AREs in the CDE-fold, independent of their thermodynamic instability. (A and B) CD melting curves of CDE1 and CDE2, their respective stabilized constructs (GG) (A) and after addition of equimolar amounts of ROQ domain (B). Respective sigmoidal fitting regions are indicated in orange. Melting points and their errors are indicated as obtained from the fitting procedure. (C and D) Imino proton spectra of the non-stabilized CDE1 (C) and CDE2 (D) at room temperature alone and in complex with different ratios of ROQ domain. As a control, the same region is shown for the ROQ domain alone in order to indicate protein-derived signals. See also Supplementary Figure S6.