Figure 4.

NEAT1 contains abundant G-quadruplex-forming sequences. (A) Quadruplex-forming G-rich sequences (QGRS) were predicted along the length of NEAT1 using QGRS Mapper. Predicted QGRS are shown mapped to NEAT1 (upper track). The subset of QGRS used in this study are identified by starting nucleotide position relative to the 5′ end of NEAT1 (lower track). (B) Circular dichroism spectra of NEAT1 G-rich RNAs annealed in the presence of 100 mM KCl, grouped as follows: QGRS with higher G-scores (left) and lower G-scores (center and right). (C) Circular dichroism spectra of NEAT1 G-rich RNAs with highest G-scores annealed in the presence (solid line) and absence (dashed line) of 100 mM KCl.