Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The possibility to perform sequential multiplexing was demonstrated by using two different circular template DNA strands, detected with Cy3- and Cy5-conjugated detection oligonucleotides, respectively. Displacer oligonucleotides were used to remove the staining of previously imaged RCPs via toehold-mediated DNA strand displacement, and in the same reaction new RCPs to be imaged were being stained. Four destaining and staining cycles are shown.
