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. 2020 Jun 4;48(13):7154–7168. doi: 10.1093/nar/gkaa474

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Antisense induction by Cdk9 inhibition and htb1-K119R is independent of H3K4me and enhanced by spt5-T1A. (A) Antisense transcript levels at cdc2⁺ and erg32⁺ loci were quantified by strand-specific RT-qPCR in indicated strains (JTB425, JTB508, JTB386, JTB408, respectively). Error bars denote S.D.; ‘*’ denotes significant differences (P< 0.05; paired t-test) from the DMSO-treated cdk9^as strain (whose value was set to 1; n = 3). (B) As in (A) for the indicated set of strains (JTB443, JTB444, JTB738-2, JTB736, respectively).